I have a DNA sample which is a mixture of fragments ranging in size from 100bp to 15kbp. I am interested in performing Nanopore sequencing on this sample, hopefully retaining information on both sequences and sizes of fragments. However, will the sequencing work properly with such diverse sizes of fragments? Is it better if I fragmentate the DNA to have a narrower distribution of fragment lengths to sequence? Thanks in advance for your feedback!
For Nanopore, the longer the DNA fragment, the better the sequencing.
100 bp is toooooo small. Of course you can use this kind of DNA for sequencing, but this would mean generation of useless data and consuming the life of pores.
Best would be extracting the DNA which yield longer and uniform fragments. Size select the DNA to remove lower sizes.
Is it better if I fragmentate the DNA to have a narrower distribution of fragment lengths to sequence? Thanks in advance for your feedback!
>> Not in case of Nanopore
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