Drug inhibits protein, but the specific gene also get no or lower expression after 24hrs of incubation ?

An enzymatic functional protein has been targeted using small molecule compound. We succesfuly found that the compound has inhibited the growth of the organism. But, we found mRNA for that...

03 April 2018 818 4 View

Journal Citation download for MS office word ? Google scholar not working ?

HI all, This is a basic clarification, where I generally copy citation from Google scholar (typing title of article, and search to download the citation with API and other formats available to...

03 April 2018 6,706 2 View

Can any one explain about Ampure beads ?

During NGS library preparation or any Bead based DNA washes carried out to remove below threshold size DNA based on the concentration of Ampure XP beads used in the respective steps. Still I...

11 December 2017 3,552 0 View

CRISPR system in Pseudomonas ? acquired or Innate ?

Recently I found there are many CRISPR genes among my Pseudomonas aeruginosa genome study. My query is whether these could be adapted from any viral or other microbes or it is innate in some of...

07 August 2016 8,751 5 View

Efflux pump detection by PCR targeting MexAB-oprM like genes is really useful or QPCR based expression of efflux gene is necessary?

Dear researchers, I have identified the presence of efflux pump genes in only 65% of Pseudomonas and absent in 35% of Pseudomonas strains. Where detection of the gene by PCR targeting MexAB-oprM...

10 November 2015 911 1 View

How can I confirm the presence or absence of a protein after drug treatment using Western blot?

As per my research, drives me to inhibit a particular gene which codes for a protein involves in multiple functions. After treating the cells with my designed drug against that protein, i have...

05 June 2015 9,716 7 View

Dear Scientists, I would like to inhibit a growth or particular gene of bacteria (Ex. E.coli) using Small interferring RNA ?

Dear Scientists, I would like to inhibit a growth or particular gene of bacteria (Ex. E.coli or P aeruginosa ) using Small interferring RNA . Can you suggest me a good protocol - related...

05 June 2015 5,767 0 View

Suggest me a journal with above 3 impact factor- Free/or below 700 Usd publication for NGS based study?

Suggest me a journal with above 3 impact factor- Free/or below 700 Usd publication for Microbial genome sequencing -NGS based study? Especially Review period with in short duration (1-2...

01 February 2015 904 2 View

Can anyone suggest a protocol and recipient strain for conjugation experiment?

Dear scientists, I have an enormous number of pseudomonas aeruginosa drug resistant strains, I would like to analyse the gene transfer ability of those bacteria through conjugation experiment. As...

31 December 2014 6,067 10 View

Integron and its explanation in detail needed, what is ISKpn4a in integron cassette?

11 December 2014 6,638 1 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

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02 March 2021 3,060 3 View

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02 March 2021 932 1 View

APA 7th In-Text citations with two references with three authors with the same surname, but four different author?

I have an issue with APA 7th in-text citation. II have two papers where the first three authors are the same, and the fourth is different. I am using the Mendeley desktop app, and automatically it...

02 March 2021 790 3 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

How to write a scientific report?

We are analysing scientific reports at University in great detail, so I wanted to find a scientific report about how to write a scientific report. Including detail such as what information to put...

02 March 2021 7,602 4 View