A resazurin-based assay was produced with ampicillin against E.coli , negative control was just LB media and E.coli and the positive being ampicillin and E.coli, with resazurin for the readings. Plate readings were taken initially and then every 30 minutes for 2 hours. The only data I have is the plate readings and the initial concentration of ampicillin added. How would I work out the final concentration of ampicillin?
Karen A. Darbinyan
Hello, same topic discussed here
Good Luck!
https://www.researchgate.net/post/Is_there_a_protocol_for_a_second_immunostaining_of_organotypic_hippocampal_slices
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