How to wash Cryptosporidium from sucrose residues after sucrose flotation?
Put the Cryptosporidium in a dialysis bag or cassette and dialyze against a suitable medium to remove the sucrose.
Does anyone know what is the ova called? It was from a water sample and sized 145.17 x 102.68 um and the second image sized 147.71 x 87.92 um. I am suspecting Fasciola hepatica or Fasciola...
01 February 2018 4,719 4 View
Hi, what is the difference between these two broths, Membrane lauryl sulfate broth and lauryl sulfate broth? Can both be used for membrane filtration method for coliform?
31 December 2016 7,764 0 View
What is absorbent pad? I tried to look from suppliers, they do not have the item. Can it be prepared by ourselves? This is for membrane filtration method for coliform.
31 December 2016 9,156 0 View
I supposedly need to use Iron sulphate and dilute it with water. How can I prepare the solution if I only have Iron sulphate-7-hydrate?
31 December 2016 9,044 5 View
Hi, I have GoTaq DNA Polymerase with concentration of 5 u/uL. How can I make 2.5U Taq polymerase if I want to prepare 50 uL total reaction mixture?
10 November 2016 921 7 View
Hi, I am having problem on how DNA is quantified by using optical density. Can anyone help me with the procedure? Most of protocols online suggest to use nanodrops. I do not have nanodrop...
10 November 2016 6,577 4 View
Hi, I did Ziehl Neelsen staining for Cryptosporidium, however the color of both Carbol fushin and methylene blue are not retained on the slide. The stains seemed not so strong and not effectively...
09 October 2016 1,553 16 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?
03 August 2024 7,829 0 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
I am currently working on a project that involves extracting cell membranes, for which we disrupt the cells using sonication. During the initial extraction process, we add protease inhibitors to...
30 July 2024 7,077 1 View
Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....
29 July 2024 5,007 0 View
I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...
28 July 2024 366 3 View