Hi,
I did Ziehl Neelsen staining for Cryptosporidium, however the color of both Carbol fushin and methylene blue are not retained on the slide. The stains seemed not so strong and not effectively stained the sample. Even observed under microscope, the slide was just clear. Is it because of I did not include phenol in Carbol Fushin solution? Or something wrong such as I rinsed the smear to strong?