How to use VCFtools?

More Benjamin Karikari's questions See All

What is the biochemistry of acidic solvents for phenolics extraction in strawberry?

I am using the Folin-Ciocalteu method for analysis of total phenolic content in ripe strawberries. I've read several protocol that suggest a methanol/H2O/formic acid (80:19:1) extraction solution....

22 February 2021 6,560 3 View

Are there any open-source datasets containing physiological information about exercises to be used for an OR-model?

I have just recently started a new "weekend project" in addition to my master's studies and I am looking for a data-set. I would like to use some Operations Research to design an optimal gym...

22 February 2021 8,023 1 View

How reliable is estimation of copy numbers by absolute qPCR?

Hello, I would like to compare the expression levels of several genes in insects, so I prepared standard curves and estimated copy numbers of each sequence in my samples. But for one of the genes,...

11 February 2021 8,421 13 View

Antibody fragments after Dha (Dehydroascorbic acid) re-oxidation?

Hi, I am trying to re-oxidize the disulfide bridges of an antibody with DHA. I always have some good amount of HL fragments (heavy + light chain fragment), despite varying reaction times, pH,...

31 January 2021 9,495 2 View

Please, any real time simulation tool recommendation for islanding detection and control in DG to me aside MATLAB as it keeps freezing and very slow?

I am currently working on islanding detection and control in distributed generations. I would kindly ask that you recommend a real-time simulation tool that helps me in this study. I have tried...

13 January 2021 3,626 3 View

Can decolonial theory speak to human rights movements?

Decolonial theory tends to avoid the language of human rights due to a concern about who is included in "the human" of human rights. But many struggles today start from the language of human...

20 December 2020 7,896 3 View

Ethanol or Isopropanol for insect preservation?

Hello, I am wondering whether Isopropanol (cheaper) can be used in the same way as ethanol for preservation and storage for molecular and DNA analyses, particularly for insect samples. Any ideas...

29 July 2020 4,994 17 View

Is it okay to have R-Squared or an Adjusted R-Squared to be over 90% in multiple linear regression? Does this raise any suspicion or question?

I ran multiple linear regression by using six IVs to predict household water insecurity in rural Ghana. My output came out with R-squared and Adjusted R-Squared of .981 and .962, respectively....

10 July 2020 5,854 2 View

What is the best program to simulate temperature of the tool tip during turning (Abaqus or Ansys)?

Dear all, I would like to simulate the temperature of a tooltip during turning. What program should I use best? And does anybody know where I can find a template where I can start from? This...

28 June 2020 2,572 3 View

No title

Hi i would like to know if there are some stat methods to aggregate Results of only one intervention group between different studies of a systematic review. in fact, I want to resume a binary...

12 June 2020 5,184 2 View

Why should we choose deep learning to machine learning? Why is CNN better for image classification? Can I get Typical Answer?

What Characteristics makes CNN work better?

03 March 2021 1,458 4 View

What are some of the research gaps in machine learning and artificial intelligence in africa?

i would to know some of the research gaps in the artificial intelligence field in most african countries.

03 March 2021 6,145 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Can you share me how teacher education is managed in your respective national educational system?

I feel that the practice in teacher education in my country is below the expected performance level due to very poor management system. Hope I will learn something from your experiences.

02 March 2021 1,516 4 View

Can NFL theorem be valid in infinite search space in ML?

NFL theorem is valid for algorithms training in fixed training set. However, the general characteristic of algorithms in expanded or open dataset has not been proved yet. Could you show your...

01 March 2021 1,189 3 View

How to calculate Total Flavonoid?

I analyzed flavonoid total spectrophotometry, the procedure as below: 2 ml supernatan added 5 ml dd.H2O, then added 0,15 ml NaNO2 5%, 5 minutes later added 0,15 ml AlCl3 10%, After 6 minutes,...

01 March 2021 6,722 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Yousif Yaqoob Shahtha

I do not know how

Nikita Zrelovs

Greetings,

It is not clear from your question what exactly is your data and what are you trying to get rid of. If you already have a list of SNPs/Indels for your "very large genomic sequence" in the variant call format (*.vcf), consult the official documentation of the tool (https://vcftools.github.io/man_latest.html) to learn about the features and usage.

Ingrid Del Valle García Carreño

http://vcftools.sourceforge.net/man_latest.html