I'm doing Surface Plasmon Resonance for a series of samples with different concentration starting with lowest concentration. Binding is so strong that I cannot reach the baseline after washing step with running buffer. Each time just part of binding dissociate. And then I test the next concentration. Several regeneration solutions have been tested but nothing could bring the signal to the baseline.
I use a Reichert SPR device and use Scrubber for analyzing the data.
How can I interpret these data in Scrubber, without a complete dissociation and/or regeneration?
Any thought would be highly appreciated.
Güçlü bir bağlanma için yüzey plazmonu kusursuz olursa scrubber rezonans ile doğru orantılı olarak çalışır. Bir süre sonra rezonans için gerekli şartlar sağlanıyor ise scrubber ve yüzey plazmonu birlikte çalışır. Ortamın tam verimle çalışması demek üçünün birlikte tam verimle çalışması demek olur.
Hi Nelly. I think it is going to be difficult. Without regeneration and complete dissociation you are basically performing a so called single cycle kinetics experiments which is a patented approach and therefore, data analysis is not available. You could run low but non-zero concentrations in between your concentration series and use TraceDrawer. Alternatively, you could use Interaction map for data analysis. Best, Jos
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