Nelly Zolfigol

3 Questions 2 Answers 0 Followers

Questions related from Nelly Zolfigol

How to use Scrubber for Surface Plasmon Resonance analysis for strong binding?

I'm doing Surface Plasmon Resonance for a series of samples with different concentration starting with lowest concentration. Binding is so strong that I cannot reach the baseline after washing...

14 September 2023 2,534 2 View

TCEP or TCEP+HCl salt for reducing thiol-modified oligos?

I have a series of thiol-modified aptamers in their oxidized form and want to reduce them using Tris [2-carboxyethyl] phosphine aka TCEP. As far as I have noticed, this chemical is sold in its...

02 August 2023 5,324 2 View

How to measure the excitation and emission wavelength of a fluoroscent material by a microplate reader?

I have a fluorescent sample and want to measure the excitation and emission wavelength using the Synergy HT BioTek microplate reader. I have tried spectrophotometer ND1000 and 3300, but want to...

31 May 2023 3,807 1 View