Which one I should choose in Abaqus ?

Hello, plese give me your idea! I simulate one slab concrete affected by temperature at top and bottom surfaces. after that, have a static load distribution with small area put on top surface. I...

12 January 2021 5,769 4 View

Is there a generalized method to derive current-mode model of any DC-DC converters?

Hi all, As new DC-DC converters are being proposed, associated control method should be proposed and based on the small-model signal analysis of the DC-DC converters. Using average space state...

06 January 2021 7,247 3 View

Analysis and implementation of Kalman filter in fixed-point arithmetic?

I would like to implement a Kalman filter for tracking applications for IoT nodes. I cannot do floating point on our nodes but only fixed point. Could you recommend some good references?

23 December 2020 4,813 3 View

Does crystallinity degree, (and crystalline phase) of a polymer affect its surface energy and adhesion?

I have several PLLA electrospun nanofibers with different crystallinity degrees and also different crystalline phases and I am measuring the adhesion force by AFM-based techniques. I wonder if we...

28 November 2020 3,269 5 View

How to avoid protein precipitation during Ni-NTA binding step?

Hi everyone, I am purifying an 18 kDa protein (his-tag). The protein expresses very well and I am able to get a clean 18kDa band in SDS Page gel after purification (attached picture). However,...

11 October 2020 3,189 6 View

Whether analysis with CB-SEM by AMOS will have better results comparing with PLS-SEM?

With your experiences, which one will have better results, CB-SEM or PLS SEM?

15 September 2020 8,092 5 View

Is there In-plane anisotropy of Amorphous CoFeB thin film?

I made CoFeB thin film on PMMA. The film thickness is from 100 nm to 10 nm. To measure magnetic properties, an M-H loop according to the angle was obtained using a VSM. However, the graph by angle...

27 July 2020 1,480 3 View

What resources or articles would you recommend for xenotransplantation procedures?

According to WHO, "the categories of xenotransplantation procedures include the following: Solid organ xenotransplantation; cell and tissue xenotransplantation; extracorporeal perfusion...

03 July 2020 6,962 1 View

Compare Curcuminoids analytical result between HPLC and Spectrophotometer ?

My problem now is when I analyze turmeric extracts (mostly curcuminoids) with HPLC, the results show 3 peaks of BDMC, DMC and curcumin. However, with spectrophotometer results, it just showed the...

04 March 2020 594 7 View

How to determine intention to use a new technology?

Hello, I am doing a research on new technologies and I would like to know how to determine the percentage of people that intend to use a new technology. I know about models like TAM, UTAUT, UTAUT2...

26 February 2020 1,381 10 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Tooth extraction and subsequent dental implant placement in Sprague-Dawley rats induce differential changes in anterior digastric myofibre size and ?

01 March 2021 7,254 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

Has anyone tried to scan fluorescence IHC slides with microarray scanner?

I have an old slide microarray scanner (VIDAR Revolution 4200) and would like to give it a "second life", as IHC scanner array (fluorescence dyes), FISH over tissue or similar. Has anyone tried...

24 February 2021 9,210 1 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

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22 February 2021 9,932 11 View