"Plasmids were co-transfected with lentiviral packaging vectors into 293T cells using Lipofectamine 3000 according to the manufacturer’s instructions", but there is no details about how to use the Lipfectamine. Is their anyone have had done it ?
Lipofectamine is a very expensive way to transfect for LV production but it's easy - just read the manufacturer's instructions - it's made by Life Technologies/Invitrogen