Hi Laure,

I hope you're doing well. I’ve put together a detailed breakdown to help clarify the process. I apologize if it’s more detail than you need, but I wanted to cover all the bases. Please see the attached picture for the most detail. Here's a quick summary...

For TGF‑β1-treated cells:

• Add 0.25 µL of the 20 µg/mL TGF‑β1 stock per 1 mL of culture media to achieve a final concentration of 5 ng/mL.

For control cells:

• Add 0.25 µL of DMSO per 1 mL of culture media (without TGF‑β1) to match the vehicle concentration.

Both conditions:

• The DMSO is diluted in the culture media so that all cells experience the same concentration, ensuring that any effects observed are due to TGF‑β1 and not differences in DMSO exposure.

I hope this helps clarify; Best wishes on your project

-Janson