I need to count nerve cells in the tissue stained with Cresyl violet. Please provide me guide to make us of Image J software for cell counting. I have cell counter plugin in Image, but I don't understand how to use it.
i attached the file what i usually use to counting the cells. and of course take a look which file of picture you do have.. i mean jpeg file, png , TIFF file! usually jpeg file works very well.
Would you please like to explain the method to count different types of cells on the same slide; at the same time counting the double-stained-overlayed (e.g. GFP and DAPI) cells in the image files?