How to transfect suspension cells with Antisense Oligonucleotides?

24 June 2023 0 7K Report

I want to transfect suspension cells with ASOs. What are the reagents I need for transfection and how to check whether the cells has been transfected and what is the protocol for ASOs transfection?

Badges
Science topic

More Amjad Hussain's questions See All

State of art in natural disasters?

Are increasing the costs of disasters in the affected countries.

01 August 2024 1,794 2 View

How to make overlapping p-h diagram in EES?

I want to draw ph diagram using Engineering equation solver EES for refrigerant R134a and R1234fy and some other. I don't know how to draw the multiple ph diagram on same dome or overlapping to...

30 July 2024 647 7 View

How do I link researchgate with my pubmed publications?

I moved to a different institute and changed my email address. Somehow I lost the list of my publications. Is there a way to link to my PubMed publication list?

17 June 2024 5,312 2 View

Feeding zebrafish larvae properly, so how many grams or milligrams of Artemia should be given to 6 dpf larvae?

According to standard guidelines, experts suggest proper feeding ratios to grow the ZF healthly.

15 June 2024 4,354 1 View

How many grams or milligrams of Artemia should be given to 6 dpf larvae?

Feeding zebrafish larvae properly is a challenge if we need to grow them. According to standard guidelines, how many grams or milligrams of Artemia should be given to 6 dpf larvae?

13 June 2024 389 0 View

How can I begin quantum computing on my computer or laptop?

I am interested in designing, developing, and testing algorithms on my laptop or local machine. Do I require any specialized quantum hardware or an online quantum computing service? Is it possible...

10 June 2024 2,917 3 View

Tensile Strength of Cement Mortar ?

Can anybody recommend the tensile strength of cement mortar? Can ASTM C307 be used to test for mortar without fibers?

03 June 2024 7,550 1 View

How measure tolerance factor of BaSrCaTiO3 perovskite?

for ABO3 if only A & B then we used t=rA+Ro/sqrt2(rB+rO) kindly explain for BaSrCaTiO3 ?

31 May 2024 2,036 0 View

Normality test of DV for multiple regression is violated. Can I still carry on with multiple regression? How can I report it?

I carried out a normality test on my DV and turns out it is significant hence violating the assumption. Do I carry on with multiple regression or what else can be done in this case?

30 May 2024 963 2 View

How to conduct comparative study of refrigerants with one heat pump model as they have different saturation pressure and temperature?

I have modelled a refrigeration system using finned and tube heat exchanger and adopted the tube by tube method. I chose the R134a as a baseline refrigerant and developed the model according to...

30 May 2024 8,152 12 View

Why is my ASO degraded quickly on agarose gel?

I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...

06 August 2024 3,130 2 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

Issues with Protein Expression in Transfected cells?

Hello I am trying to create a stable cell line in HEK293 via Lipofectamine 3000 transfection. My plasmid is a CD63-IL10-GFP construct with Puromycin resistance. I am successful with the...

30 July 2024 6,648 1 View

How to retain the a GFP tagged gene expression in stable cell line?

Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....

29 July 2024 5,007 0 View

Does anyone have experience electroporating SKOV3 cells with a large plasmid using Neon transfection?

I have been trying to electroporate SKOV3 cells with a large plasmid (11kb) without much success. Any tips?

29 July 2024 3,229 1 View

Why cannot i find my protein on cell surface after antibiotic selection of expressing plasmid?

I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...

28 July 2024 3,178 3 View

Can I use Polyjet after its expiration date?

I have a Polyjet that has passed its labeled expiration date for 1 year and I'm going to re-start transfection experiments. It is really needed to change it? Transfection efficiency may be lower?

23 July 2024 8,059 1 View

How to transfect circular mRNA to T cells?

Hello! We are using LNP to deliver circular mRNA into T cells. However, compared with linear mRNA, the transfection efficiency and protein expression duration are similar. Are LNP generation or...

21 July 2024 4,322 1 View

Transfection of A549 using lipofectamine 3000?

Im trying to transfect A549 cells using lipfectamine 3000. im using a 96 well plate with 1microgram plasmid DNA in each well . should i remove the culture media or change it or decrease it befor...

21 July 2024 7,610 2 View