Hello,
I have been trying to do a lentiviral transduction of mouse primary CD4+ T cells with little luck. We do not see any expression and cells begin to die out.
Current protocol:
Isolate CD4+ T cells, transduce on day 7 with spinoculation (1000G, 1hr)
Thank you!
Oliver Liang
Hi Meisha,
Have you checked whether the cells survive and are sufficiently activated? Just wondering if you have an untransduced control that also starts to die out around the same time (in which case the problem can be with ex vivo culture).
The following protocol might be relevant for your interest, if you havent read it (An optimized protocol for the retroviral transduction of mouse CD4 T cells - PMC (nih.gov))
All the best with your experiment!
Oliver
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