How to test purity of cultured macrophages with flow cytometry?

More Ruby Nieh's questions See All

I am new to cell culture technique and reading papers on cell culture.Is there any criteria for selecting certain cell culture (cell line)?

I need to select a cell type to start my experiment. My experiment is on female genital tract infection and I intend to see cytokine expression. I have read about J774 but I do not know why to use...

09 August 2020 8,504 3 View

Need help- how to use primary and secondary data in same paper in regression?

Actually am trying to use regression for my paper where am drawing primary data questionnaire(independant)from few universities students and trying to establish relations with student...

02 May 2020 7,097 3 View

Can anyone help me convert the concentration of human anti-hepatitis B antibody (Hepabig) from IU/ml to mg/ml?

I am using Hepabig brand which only states 100 IU/0.5 ml concentration of antibody but no mg/ml immunoglobulin concentration estimate.

05 March 2019 5,808 15 View

How to tell apart primordial germ cells from EG cells?

Is there any method that can tell apart EG cells from primordial germ cells? By morphology or surface marker, gene expression, et cetera. ?

11 November 2018 2,117 3 View

Is there a simple method to use pslegend (GMT) to create a legend for 2-D plot?

I am new for Generic Mapping Tool (GMT), but I would like to work through a few simple examples of generating legends (ie. multiple points or lines plotted on a single figure using psxy). In the...

26 April 2018 8,197 3 View

Hi, is it possible to observe increase in cell viability of normal fibroblast cells (NIH3T3) with increasing biosurfactant-coated iron oxide NPs?

I have repeated the experiment twice (in triplicates/experiment) and have observed increase in viability with increased nanoparticle concentration (100 ug/ml-1 mg/ml). Can anyone please help me...

15 March 2018 8,589 2 View

How to use high precise geoid for mineral exploration?

Recently, many scientific applications such as: In geology, the inversion of the geoid is used for Petroleum Exploration. I am looking for studies and research in this field

01 January 2018 2,539 4 View

Is there big difference between the monophasic stimulation and biphasic stimulation on the neuronal secretion of neurotransmitter

Is there big difference between the monophasic stimulation and biphasic stimulation on the neuronal secretion of neurotransmitter? I want to detect the stimulated DA in the striatum when...

26 May 2017 6,683 2 View

What methodology would you recommend for analyzing I Daniel Blake and an observational tv reality show such as Benefits Street?

As a social scientist, the world of film analysis is new to me and I am interested in exploring how lone parent wellbeing is portrayed in I, Daniel Blake and then contrasted with an observational...

25 April 2017 5,443 5 View

Does anyone have experience with variations in infection efficiency?

I am using lentiviral vectors (2nd and 3rd generation) containing a fluorescent labeled backbone (hOSKM). After transfection of HEK239-T cells, were I could see a clear fluorescent signal after 24...

18 September 2016 6,219 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View