How to test purity of cultured macrophages with flow cytometry?

More Ruby Nieh's questions See All

How do creative and critical thinking affects the development of 21st-century competencies?

28 January 2024 8,078 5 View

What is the influence of the satellite's attitude on the accuracy of the instantaneous range?

Assume I have the coordinates of satellite xs (t) and ground station xg (t) in the International Terrestrial Reference Frame (ITRF). Also, the attitude of satellites in the form of quaternions is...

10 December 2023 8,820 0 View

In focus reduction neutralization assays, there is a lot of variation between the virus phenotypes between each well. Can anyone explain this?

In focus reduction neutralization assays, we apply a virus and serum mixture to a monolayer of cells. After infection, incubation, fixing, and staining we count the number of foci (formed from...

07 June 2023 4,211 1 View

How can the divergence between the transmitting laser and the retroreflectors be minimized in Satellite Laser Ranging (SLR)?

The general characteristics of Satellite Laser Ranging (SLR): The photons returning are usually fewer because the transmitting laser and retroreflectors both have a divergence. This means that the...

10 May 2023 9,289 10 View

Does stakeholder pressure influence the extent of corporate water disclosure in water-sensitive industries?

29 April 2023 6,224 1 View

Is the frequency drift value constant, linear, or nonlinear variation in the case of an atomic clock?

In an atomic clock: instability in the oscillator and environmental changes can cause frequency drift, although it is often hard to identify between drift and oscillator aging. So, is the...

16 March 2023 2,774 4 View

Shall I combine my analysis and discussion for my dissertation?

Hi guys, I wanted to ask a question about my dissertation. It is an IPA study and I am wondering if I should combine the analysis and discussion section or leave them separate. Most journals I...

08 March 2023 2,490 3 View

Can I remove a participant from my qualitative study?

Hi guys, I am currently analysing my interview data conducted for my dissertation. I am obviously a novice researcher so would appreciate some advice. I have four interviews conducted and one is...

22 February 2023 10,019 2 View

How to implement management strategies in VORTEX for PVA analysis?

I want to test the efficacy of a head starting program on a specific lizard species, but I am unsure of how to do this correctly on the simulation software Vortex. I believe I have to create state...

08 February 2023 2,197 1 View

Phenomenology or grounded theory approach?

I am looking to conduct a qualitative study on the role of women's self esteem whilst they're at work. I know your research question should guide your design but I am struggling to choose which...

13 October 2022 5,518 3 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Why do we equate male and female arousal?

Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)

05 August 2024 9,537 2 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

How can I get my Granzyme B flow cytometry stain to be consistent?

I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...

01 August 2024 7,677 2 View