Hi Everyone, Hope you are doing well, currently i am working on ARMSPCR study to detect some mutation, but I am unable to understand how can I take a positive control for my study if earlier i wont have any positive patient sample for that specific mutation. Is thetre any way to make a PCR product as positive control ? if yes whaths the way? please respond my question ane guide me on this.
Thanks for your guidence in advance.
You would have to order PCR primers with a "mismatch" to amplify a PCR product that has the SNP you need. Then, clone said PCR product into a plasmid. Transform into bacteria & you'll have the ability to create more control DNA that you'd ever need.
Good luck!
Hello Mam Thanks for your guidence, I have all the required primers with me all tetraprimers which need to detect the mutation should i go with the same primers Katie A S Burnette
No, those primers will not work to create a positive control. Diagram it out and you'll see why not.
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