I have tried to modify gelatin with methacrylic anhydride (MA). However, when MA was added into gelatin PBS solution, it will form milky emulsion and a kind of precipitation. after dialysis, the precipitation can be reduced in some extent, but still exists.
can I ask whether this is a normal procedure or what should I do to avoid the precipitation?
Cody O'Keefe Crosby
You need to add the MA VERY slowly, or it will precipitate back out of the solution. We add dropwise with a glass pipette and have had good results.
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Filip Hasecke
Just a follow up on this question. I've had the same issue now and wanted to ask: Did it affect down-stream applications? Did you remove the precipitate by centrifugation? Or did you simply start over?
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