Is RIPA Buffer from SANTACRUZ is suitable for NUCLEAR protein extraction and downstream western blot analysis using ABCAM Y-69 antibody?
We have set the voltage profile between 2.7 to 4.0 voltage to charge the cell. As we charge the cell it doesn't reach to 4.0 but flattens at 3.2 V.
26 November 2023 2,327 0 View
Working on a sub-basin of a large Watershed and when I run the uci file in HSPF, I get this error " ERROR/WARNING ID: 238 12...
19 August 2022 3,744 0 View
I am looking for the analysis of target genes of E2F. There are many genes mentioned in textbooks and various publications. However, I want to select major 4-5 genes targeted by E2F? Thanks
24 November 2021 1,861 0 View
Previously, to perform a rheological study on the collagen matrix that we tried to make we used 2000uL of Collagen, 100uL of 10X PBS, and 13uL of NaOH. It formed the gel. For performing a better...
13 May 2021 1,273 1 View
I came across a paper on Incipient Fault Location Algorithm for Underground Cables which was published in IEEE on 3 May 2014 from which I could understand the method of anticipating faults using...
05 March 2017 5,351 3 View
If one can perform 'Head-Shake Test' (HST) to examine Vestibular deficit after a child has suffered concussion, specially during the acute stage (1 week post injury)? Will there be any risks...
17 March 2014 6,684 5 View
I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.
11 August 2024 9,101 4 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!
11 August 2024 3,770 4 View
Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...
10 August 2024 7,180 0 View
I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...
10 August 2024 7,429 2 View
I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...
10 August 2024 7,480 3 View
How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?
09 August 2024 7,718 0 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View