Pure experimental/ laboratory method will be adopted. Administration and determination of the lethal dose LD 50 on experimental animals like mouse and rabbit. And a close examination of those animals at various doses and behaviour. The results then will be extrapolated to discus the effects of Copper on humans
Is it not true that according to the (WHO) World Health Organization 70% of the world is deficient in copper? Can you give some more information on the reasoning behind the question?
If you have an area where there is copper pollution, I would begin with a population study of what diseases occur there at elevated levels compared to areas without copper pollution. From there it would be easier to direct laboratory studies to look at the mechanism by which copper might affect the pathogenesis of those diseases.
Susan - for you and others who study metal ions, I receive an email this morning about a meeting in late November 2016 on metal ions in biology and medicine. This International Symposium will be held in Mumbai and so I would encourage you to attend if possible. I would love to attend, but finances will probably not allow me go.
http://www.metalions2016.com/
Is there a high occurrence of Wilson's diseases in the population that you are looking at? This can have a profound affect on people if not treated. I have a suspicion that with this disorder there is more likely hood of other things building up in the systems of these people as well. There may be more then just copper that is building up and the mechanism that helps the body remove this may also not allow the removal of other things like Iron and lead or zinc. I think that study would have to be on the key removal issues with all metals.
Various biological essays in vitro you can use, like MTT, LDH to study cytotoxicity. You can use also genotoxiciy essay on human lymphocyte described in some my references, or some other kind of immune cells.
Hi everyone,Thank you very much for support anf informations
regards susan verghese P
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