Hi,
I want to study drug- receptor interactions. Specifically, I would like to know to which receptor my drug of interest binds. Please help me with the methodology to do so...
One method which I hypothesised was to label the drug chemically with some tag and then incubate the cells with this tagged drug. Then pull down the tagged drug and its interacting molecules and run on SDS PAGE. Check using specific antibodies for the hypothesised receptors.
But is it possible to chemically label the drug. Won't it change the properties of the drug. If not, then please do tell me which company provides such services of tagging the drug.
This method can be wrong. So please do tell me the correct way of doing it
Thanks in anticipation.
Cheers,
Apurva Agrawal
@Ankit and @Saeideh: Thanks a lot for your help. SPR imaging is a good solution. The next problem is to find such services nearby and at affordable cost
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