Hi,
I am planning to study cognitive impairment in PD. I will use rotenone as a neurotoxin and will infused it into substantia nigra and ventral tegmental area in SD rats, to induce PD and cognitive impairment. I choose ventral tegmental area because of its intensive projections to the cognitive domains such as prefrontal cortex, hippocampus, amygdala. I wonder that how reliable model is this? Do you have any suggestions or directions to study cognitive impairment in my rotenone-infused PD model?
Thank you.
Interested in the study. Pending results. Courage at work!
I suggest that "novel object recognition test" or "morris water maze" for hippocampus, "Cued fear conditiong and tone test" for amygdala, and "T-maze" for prefrontal cortex.
See Stan Floresco's work on behavioral flexibility and dopamine in the PFC. There are many ways to get at aspects of behavioral flexibility. Showing a rotenone effect via VTA infusion would be interesting. Regarding the reliability of rotenone infusions as a model, my guess is that it will not be as reliable in the VTA relative to the SN. It's rarely administered in the VTA alone.
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