Respected Sir/Madam,
I am working in remediation of textile dye effluent and toxicity effect in fresh water fish? I first use chemical remediation for de-colorization of effluent (primary treatment) and use diatom Odontella aurita, for reducing the effect of the chemical, and toxicity removal ( secondary treatment).In the treated effluent,I can make the fish survive for 7 days ( proving the treated effluent is less toxic). Now I am unable to determine the methodology of how the diatom work. I can see white sediments after diatom treatment.
The problems I face during characterization, of my diatom, is it gets mixed with the sediment after remediation, and unable to segregate. So I find it difficult to get sample for IR, SEM studies. I get only one gram of sludge for 10 liters, making me difficult to characterize the sludge too.
Can anyone please suggest me if, there is an alternative to find the mechanism on how diatom detoxify's primary treated effluent. One hint I have is diatom takes 48 hours for remediation and shows no reproduction in the effluent. Please Help.