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Questions related from Bhagya Deepachandi
What are the other recommended methods for concentrating plant extracts except to the rotary evaporator and freeze dryer?
25 October 2024 3,910 5 View
Please suggest some reliable tools for computing multiple sequence alignment and generating heatmaps?
23 November 2022 6,592 3 View
How to select a model for constructing a phylogenetic tree using maximum likelihood method?
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I have a patient group with two different categories of clinical features (say clinical group 1 and clinical group 2). And there are two diagnostic assays to analyze this patient group (say assay...
10 April 2020 9,896 2 View
Is it necessary to boil dialysis tubes in EDTA/sodium bicarbonate before using? or is it enough to boil it in distilled water? What is the recommended protocol for preparing dialysis tubing?
27 May 2018 4,683 6 View
Usually I am doing PCR with 0.5 ul of Taq polymerase (abm) per reaction. Since I wanted to increase the sensitivity of target amplification, I tried PCR with 1.0 ul of Taq. It gave some bands in...
26 March 2018 395 5 View
Leishmania talentorae is known as non-pathogenic parasite to humans. Can it co-exist with other different pathology? What are the optimal conditions for growing Leishmania talentorae in-vitro?
24 December 2017 6,159 3 View
I have found two commercial ELISA kits for detection of human leishmaniasis. 1. Human Anti-Leishmania IgG ELISA Kit (ab108749) by Abcam 2. Leishmania IgG ELISA Kit (KA3295) by Abnova Both are...
03 April 2017 6,433 1 View
I am facing the a problem in my SDS page gel. protein marker and samples not get well separated. All samples were stop separating at the middle of separating gel (protein marker also). Dye front...
19 October 2016 3,574 11 View
In what temperature we should store a lyophilized serum sample for long term greater than one year? Is there any effect from keeping it in -20 C?
22 October 2015 1,974 5 View
What is the maximum time we can store blood before the tests, FBC and total protein? and at what temperature?
06 June 2015 6,371 3 View
I have done the first step of optimization of indirect ELISA. It is "optimization of antigen coating". Coating and other procedures are correct. The problem is with evaluating the absorbance...
05 April 2015 5,681 6 View
I used positive and and negative serum for optimizing an ELISA, but there is a difference between each sample. The total antibody content is different from person to person. So, it is not...
15 December 2014 3,421 12 View
What is the ideal temperature for storing serum and lyophilized serum for a long term?
23 November 2014 6,594 4 View
Does anybody familiarize with the freeze thawing method to isolate proteins? what is the normal yield of protein we can get from it?
30 October 2014 9,798 3 View
Is it TMB or ABTS?
02 October 2014 6,298 11 View
Immunoblot assay make the proteins be denatured? Is that suit for every antigen-antibody interactions?
06 September 2014 7,133 3 View
Manual purification of proteins using anion exchange chromatography
31 August 2014 7,502 4 View
What is the observed range for molecular masses of leishmanial donovani antigens?
24 August 2014 1,798 1 View
