We have received a new batch of SHSY5Y from the suppliers, and the cells are growing in clumps/spheres even after trypsinization.
Is there a way to control/stop it?
I just read this, and changing the brand of serum of adding more serum (>10%) may help?
Article Human Cells Grown With or Without Substitutes for Fetal Bovine Serum
Hello Sanjukta Ghosh
The clumps that you are referring to is the undifferentiated SHSY5Y cells which tend to grow in clusters and may form clumps of rounded cells on top of one another in the central region of a cell mass. At the edges of the cluster, cells begin to extend short neurites. In the undifferentiated form, SHSY5Y cells are characterized morphologically by neuroblast-like, non-polarized cell bodies with few, truncated processes.
You may obtain more information in the below attached paper. You may refer to Fig 1. On differentiation SHSY5Y cells do not cluster and have a more pyramidal shaped cell body. See Fig 2.
Article Considerations for the Use of SH-SY5Y Neuroblastoma Cells in...
Best.
Thanks Malcolm Nobre.
Differentiation is definitely my next way out. But I still have some experiments to be carried out with undifferentiated cells. I have seen adding extra serum, 11% total helps the cells spread out of the clumps.
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