15 Questions 9 Answers 0 Followers
Questions related from Sanjukta Ghosh
Hi there, I am really curious to know by what ways researchers from different background, especially those working in Computer Science or Computational biology, protect their research progress...
22 April 2025 2,283 4 View
What are the purchasable ligand databases which are currently also updated. I have been looking into Zn purchaseable db through Pharmit server, it is really not updated and the ligands are no...
02 December 2024 3,456 3 View
Hi there, My question is: What standard curves should be used while estimating Tot GSH and GSSG by kinetic method using GR enzyme mediated recyling with DTNB chromophore? Actually I am following...
02 August 2024 8,775 1 View
From the definition of cell line, they are indefinitely propagated/subcultured. However, in practice beyond a certain passage number, they are said to loose characteristic gene profile, for e.g....
11 June 2024 6,939 1 View
I have received passage 29 from our vendor (NCCS, Pune). The cells are slightly different from the previous batch, they form neurospheres, and on adding 12% instead of 10% they show 90% monolayer....
11 June 2024 5,297 0 View
Hi there. I am working with SHSY5Y. I am using passage no. in its early 30s. I am treating it with a well establishe drug that is reported to promote growth in these cells by redcuing the amount...
19 May 2024 1,564 0 View
The human protein model predicted by AlphaFold2 is not completely passing Save Server (It is not clearing Verify3D), instead the mouse AlphaFold2 model is able to pass all tests. Can the...
13 May 2024 585 0 View
We have received a new batch of SHSY5Y from the suppliers, and the cells are growing in clumps/spheres even after trypsinization. Is there a way to control/stop it? I just read this, and changing...
04 April 2024 8,665 2 View
Hi, This question has been asked by many scientists in different ways, my question might be a modification of the same. I have been analysing RNA-Seq data on Galaxy with Limma-voom and used two...
02 February 2024 4,557 0 View
I have three RNA-Seq datasets of the same tissue and want to analyse them on Galaxy. My initial literature survey gave me the idea that I can merge the three datasets if they are from the same...
09 January 2024 7,405 7 View
I am working with SHSY-5Y and using NEST-made tissue culture plates. I am looking for some companies that may support stronger adherence to these poorly adherent cell type.
31 December 2023 7,179 5 View
I can't find any resources online. Can somebody suggest anything?
31 December 2023 2,279 0 View
Here are pictures of two SHSY5Y flasks maintained by two scholars. One shows shrunken discrete pointed morphology and the other has comparatively large morphology, with both skind-of-spindle...
29 September 2023 5,466 1 View
Based on my literature survey, I have seen that 24 hours for cell adhesion is recommended. The articles or standard video tutorials do not mention of attainment of morphology as an essential...
12 September 2023 9,924 7 View
Hi, I have procured N2a cells, from a lab that had maintained the cells in DMEM-HG w/ Glutamax (supplement of Glutamine). I am basically trying to capture increasing death pattern with increasing...
01 January 1970 3,112 0 View
