Sanjukta Ghosh

11 Questions 8 Answers 0 Followers

Questions related from Sanjukta Ghosh

GSH estimation assay: What is the right choice of standard?

Hi there, My question is: What standard curves should be used while estimating Tot GSH and GSSG by kinetic method using GR enzyme mediated recyling with DTNB chromophore? Actually I am following...

last month 8,071 1 View

How are cancerous cell lines generated in 1900s sold today have the same genetic profile?

From the definition of cell line, they are indefinitely propagated/subcultured. However, in practice beyond a certain passage number, they are said to loose characteristic gene profile, for e.g....

11 June 2024 6,761 1 View

Why is SHSY5Y getting killed by protective drug?

I have received passage 29 from our vendor (NCCS, Pune). The cells are slightly different from the previous batch, they form neurospheres, and on adding 12% instead of 10% they show 90% monolayer....

11 June 2024 4,630 0 View

Why is a cell line (SHSH5Y) not growing with growth promoting drugs?

Hi there. I am working with SHSY5Y. I am using passage no. in its early 30s. I am treating it with a well establishe drug that is reported to promote growth in these cells by redcuing the amount...

19 May 2024 1,375 0 View

Can AlphaFold2 predicted structure be used as template for model prediction on another software?

The human protein model predicted by AlphaFold2 is not completely passing Save Server (It is not clearing Verify3D), instead the mouse AlphaFold2 model is able to pass all tests. Can the...

13 May 2024 381 0 View

How to stop SHSY5Y neurospheres from growing?

We have received a new batch of SHSY5Y from the suppliers, and the cells are growing in clumps/spheres even after trypsinization. Is there a way to control/stop it? I just read this, and changing...

04 April 2024 8,567 2 View

Is it correct to merge different RNA-Seq datasets and perform differential expression analysis on Galaxy?

I have three RNA-Seq datasets of the same tissue and want to analyse them on Galaxy. My initial literature survey gave me the idea that I can merge the three datasets if they are from the same...

09 January 2024 7,317 7 View

Is there any special brand of tissue culture plates for loosely adherent cells?

I am working with SHSY-5Y and using NEST-made tissue culture plates. I am looking for some companies that may support stronger adherence to these poorly adherent cell type.

31 December 2023 7,098 5 View

Can you suggest online study materials for docking Iron-metalloprotein with AutoDock4?

I can't find any resources online. Can somebody suggest anything?

31 December 2023 2,213 0 View

Which of these SHSY5Y samples are healthy?

Here are pictures of two SHSY5Y flasks maintained by two scholars. One shows shrunken discrete pointed morphology and the other has comparatively large morphology, with both skind-of-spindle...

29 September 2023 5,398 1 View

Is it important to allow the cells to attain morphology before treatment in MTT assay?

Based on my literature survey, I have seen that 24 hours for cell adhesion is recommended. The articles or standard video tutorials do not mention of attainment of morphology as an essential...

12 September 2023 9,860 7 View