When I tried to stir magnetic particles suspension, all the particles got stucked to magnetic bead.
Is there any way to stirr a magnetic nanoparticle suspension on a stirrer without sticking these magnetic particles to the magnetic bead ?
You can stir them using a mechanical stirrer. Also you can use powerful ultrasonic .
hi, prashant
i also faces the same problem. you can use either mechanical stirrer or ultrasonic. but if you use the bath sonicator it will not disperse the materials due to the heavy molecular weight of ferrite samples. if you use the probe sonicator it will affect the crystallite size and morphology.
best way to disperse is mechanical stirrer.
another way is you just cover the magnetic bead by aluminium foil or any stainless steal foil. in this way you can easily separate the magnetic particle from the beads when once you remove the foil from bead
Dear Prashant,
there are two possibilities we use:
-With larger quantities you can use a KPG stirrer with slow stirring speed, ensuring, that there is no abrasion or distruction of the particles,
-alternatively you can use a magnetic stirrer with an agitator. After process you can rinse the agitator by means of a washing bottle and recover the sticking beads.
We use these techniques for our own magnetic beads, which show a very strong magnetic moment. This procedure with other magnetic beads with a lower magnetic moment is even easier.
Best, Roland
https://cube-biotech.com/products/magnetic-beads/
thanks Behrooz Tanazmaei , thanks Baskaran Palanivel ji,
thank you Roland Fabis sir..
we are using Hula mixer or similar mixer, when using Dynabeads with any sample type, in 1-50 ml tubes. not magnetic stirrer
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