Hello...
I’m using inflammation induced keratinocyte culture media to re-induce inflammatory responses in RAW 264.7 macrophages. When I transfer the media from inflammation-induced keratinocytes to RAW 264.7 macrophages, it clearly induce inflammatory responses (NO production etc.). What I want to know is, which cytokine or signaling molecule (coming from keratinocyte culture media) is usually responsible for inducing inflammation in RAW cells.
Thank you
Hello Shanura,
The release of pro-inflammatory cytokines from the keratinocytes depend on the passage number of the cells and the stimulating agents. Therefore, the comments would be more hypothetical and theoretical. Mostly, the secreted cytokines are TNF-a, interleukins (1b, 6), chemokines (CCL2, CXCL10), and interferons (beta and gamma). etc.
Yes, I agree with the above assessment. In my hands, TNF and IFN-gamma are the most potent, possibly also involving MCP.
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