Shanura Fernando

3 Questions 5 Answers 0 Followers

Questions related from Shanura Fernando

How to stimulate RAW 264.7 macrophages using pro-inflammatory cytokines?

Hello... I’m using inflammation induced keratinocyte culture media to re-induce inflammatory responses in RAW 264.7 macrophages. When I transfer the media from inflammation-induced keratinocytes...

13 April 2017 3,045 2 View

How to perform SDSPAGE for polysaccharide molecular weight distribution analysis?

I have some polysaccharide fractions obtained by DEAE-sepharose column separation. I wish to determine its approximate molecular weight by caring out SDSPAGE. However I cannot obtain nice bands....

30 November 2016 9,530 5 View

How do I solve Convergence failure - run terminated error when using DFT to optimize a molecular structure of a Fucoxanthin homologous?

Hi... I’m in need of optimizing a molecular structure of a Fucoxanthin homologous for molecular docking analysis. I tried using DFT with 6-31+G* basis set after optimizing the molecule with HF...

07 December 2015 3,437 11 View