Shanura Fernando

4 Questions 6 Answers 0 Followers

Questions related from Shanura Fernando

Does anyone have a reliable protocol for methylation analysis of sulfated polysaccharides?

Hello…Can someone kindly provide me the detailed procedure to carry out desulfation and methylation analysis for fucoidans? There are many publications, but neither of them describes how to do it...

02 August 2017 9,708 2 View

How to stimulate RAW 264.7 macrophages using pro-inflammatory cytokines?

Hello... I’m using inflammation induced keratinocyte culture media to re-induce inflammatory responses in RAW 264.7 macrophages. When I transfer the media from inflammation-induced keratinocytes...

13 April 2017 3,050 2 View

How to perform SDSPAGE for polysaccharide molecular weight distribution analysis?

I have some polysaccharide fractions obtained by DEAE-sepharose column separation. I wish to determine its approximate molecular weight by caring out SDSPAGE. However I cannot obtain nice bands....

30 November 2016 9,533 5 View

How do I solve Convergence failure - run terminated error when using DFT to optimize a molecular structure of a Fucoxanthin homologous?

Hi... I’m in need of optimizing a molecular structure of a Fucoxanthin homologous for molecular docking analysis. I tried using DFT with 6-31+G* basis set after optimizing the molecule with HF...

07 December 2015 3,440 11 View