How much cell pressure and back pressure should I apply to start for triaxial test for different types of soil?

We have recently purchased a triaxial test instrument (MATEST) for testing different types of soil. After installing software, we are facing a issue of saturation where the samples does not...

23 February 2021 7,194 4 View

How to produce huge amount of protoplasts from Fusarium graminearum?

Hi, all I am using some protocol for protoplast production from Fusarium graminearum (Fg), but I need them in a huge amount. Sometimes the protocol worked well but most of the time failed....

14 February 2021 2,286 4 View

Whom should be given the priority for vaccination for Covid-19?

Mass vaccination for Covid-19 is going to start very soon. Whom do you think should be given priority for vaccination? Senior citizens, frontline workers or students? Why?

24 December 2020 8,267 6 View

How to do Fusarium graminearum protoplast transformation using desired plasmid?

I would like to specify my question regarding the transformation of plasmid DNA into F graminearum protoplast. I am using the following reaction mixture (30%PEG 50ul, 200ul protoplast, and 20ug of...

14 December 2020 4,251 1 View

How could we redope Ni deposited PPy?

We prapare Ni deposited polypyrrol (PPy). But after Ni deposition, the conductive polymer is depoded and in CV analysis, the observed current value is very low. An advice is expected from the...

02 December 2020 9,204 3 View

Could you explain the following situations, please?

I am trying to simulate a flexible antenna on ANSYS HFSS. Here, I have used "PDMS" as a substrate and "pec" as a probe (inner conductor) of a coaxial feeding. But during simulation, it is showing...

24 November 2020 800 5 View

What technique can be used to monitor carbon dioxide in natural waters?

I am looking for a method or a sensor for the monitoring of the dissolved carbon dioxide in wastewater and natural water.

22 November 2020 3,439 4 View

Can I get an explanation for the following reason, please?

My plan is to fabricate a conformal microstrip patch antenna. I am going through the simulation works first. I already simulated the planar antenna. Now I am trying to simulate it with different...

19 November 2020 2,961 2 View

I want to determine intracellular calcium concentration of Hela cells using Fluo-8 AM? Is there any protocol?

I also want to use Spectro fluorometer to measure calcium concentration. One more thing, after treating cells with calcium dye, Should I lyse the cells or not to measure calcium concentration?...

11 November 2020 7,757 3 View

Is it ok to spin serum at 7000 rmp for ELISA?

I wand to estimate antibody in serum by ELISA. In order to obtain clear serum, is it ok to spin serum at 7000 rpm (without refrigeration) for 5 minutes? Thank you in advance.

08 November 2020 2,456 5 View

What is the most Operating System (OS) which can be INSTALL on Tiny hard devices like Raspberry Pi (RPi) hardware ?

I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...

03 March 2021 1,931 5 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

No title

02 March 2021 3,060 3 View

How can I integrate a routing protocol in a tiny device (e.g. Raspberry Pi)?

Hi Community, I'm facing the issue of integration/compiling a new routing protocol in a WSN simulator. The final goal is to successfully add, configure this routing protocol in hardware devices...

01 March 2021 9,332 6 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View