actually I am preparing hydrogels based on silk fibroin and tannic acid and it take 12 hours for formulation at 37°C temperature. but for the cell cytotoxicity test i need to prepare hydrogel under aseptic conditions .i have tried to form by adding calcium ions under aseptic condition but my hydrogels did not form .after that i also used different methods for hydrogel sterilization like placed under UV light by adding ethanol for 4 hours and also tried by adding cell culture medium but i did not get results ..please suggest me which method i need to use .. it would be highly appreciate
Sterilize hydrogels using gamma irradiation or UV irradiation, or steam sterilization, while carefully considering potential impacts on hydrogel properties like stability or degradation. Test different sterilization methods to find the method that work best for your hydrogel.
If your gel is less viscous, you can filter it using a 0.22 micrometer filter.
If at some point you obtain a dried hydrogel, why not trying steam sterilisation with an autoclave? You can adjust the temperature (better not go below 115°C to ensure a good sterilisation though) and time. As far as I know, silk fibroin degrades at around 200-250°C so steam shouldn't affect the overall properties (however, you can check by performing mechanical and/or rheological tests before and after the treatment to see if there's any difference)
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