I need to conduct accelerated ageing on corn seeds, however, seeds were infested by pathogens (I observed fusarium moniliforme, Aspergillus niger and flavus), so when place in high humidity condition, promoting the infestation itself. I have tried many combination of ethanol (70%) and sodium hypochlorite (5,10, 50%) and soaking time ranging between 2-10 minutes. The results is either infection was still occurring or no germination was observed once germination test was conducted
If anyone could advise on how to properly sterilise the seed and does not affect seed accelerated ageing, it would be much appreciated.
You should do seed viability tests before assigning seeds for germination using the flotation test (viable seeds sink). Then you could use gamma irradiation to kill microorganisms but retain viability. See:
Article Gamma radiation effects on seed germination, growth and pigm...
https://dx.doi.org/10.1007%2Fs10867-013-9322-z
Generally, Healthy and viable seeds do not infected by pathogens. Since the seeds are under accelerated ageing process and are not germinating, the seeds may partially lost the viability and getting infected. Viability test is recommended to confirm the same.
MERCURIC CHLORIDE IN LOW SILUTION IS IDEAL, HWEVER YOU MAY CONDUCT EXPERI,EMTS O VARIOIUS LOW CONCENTARATIONS AND WASH THE WITH DIDTILL WATER AND RAISE SEEDLING IN LARE PETRIPLATES INDIDULLAY
Please see the following link:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC439241/pdf/plntphys00313-0162.pdf
A few seconds with ethanol and 5 to 10 minutes with bleach diluted 10%.
- Badges
- Science topic
- Similar topics
- Agriculture
- Horticulture
- Seeds