I have few GPCR agonists and I want to know whether they produce any statistically significant ERK activation in terms of their EC50 values (Graphpad prism data) as compared with my standard agonist. My standard agonist's EC50 value is 1.6nM. Two of my ligands have EC50 values of 3nM and 55nM respectively. Could you please advise on how to determine in a rather simple way if my test compounds produce any statistically significant ERK activation with particular reference to the above given numbers?
Dear Ashgar
You would need an estimate of the variance of your data. This implies that you should determine your EC50 values in at least three independent experiments. This will give you 3 EC50 values from which you can calculate a mean and a standard deviation. You can then compare the mean of your standard agonist with the mean of another ligand, using a simple test like the t-test. You can also compare the mean of your standard agonist with the means of a number of other ligands, using ANOVA type tests, followed by post tests. Finally you can use the full data set for the EC50 determinations and test all data against each other. A good textbook on biostatistics would be
https://www.amazon.co.uk/Biostatistics-Essentials-Geoffrey-R-Norman/dp/1550093479
Best wishes for your research
Birger
Hi Asghar, I suggest that you review the publication "Dose-Response Analysis Using R" (Ritz et al 2015; PLOS ONE), It has several examples of EC50 calculations under various types of experiments.
Good luck and best regards,
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