Junaid Asghar

3 Questions 7 Answers 0 Followers

Questions related from Junaid Asghar

How to statistically compare EC50 values of my agonists?

I have few GPCR agonists and I want to know whether they produce any statistically significant ERK activation in terms of their EC50 values (Graphpad prism data) as compared with my...

08 August 2016 7,954 4 View

My agonist stock is 10mM made in DMSO. How to go about with the vehicle control for a serial dilution starting from 10uM?

The question is basically about doing the vehicle control test for DMSO in mammalian cell culture (CHO cells). A working strength of 0.1 to 0.5 % DMSO is generally advised to be safer for most...

04 April 2016 6,011 6 View

How can a malignant melanoma be induced in sprague dawley rat?

I have to induce malignant melanoma in sprague dowley rats. Prior to that, I need to suppress its immune system and, if possible, irreversibly weaken the immune system so that the rat's immune...

04 April 2014 2,007 4 View