I have to induce malignant melanoma in sprague dowley rats. Prior to that, I need to suppress its immune system and, if possible, irreversibly weaken the immune system so that the rat's immune system can not respond to inoculated malignant melanoma cells, and malignant melanoma can develop.
Any comments would be appreciated, Thanks
There is a rat equivalent of nude mice (rnu), mutant at Foxn1, which makes them immune-deficient and also hairless. It is commercially available. You could check if it is available on the Sprague-Dawley strain [it's Dawley not Dowley] .
It is better to use the B16 melanoma in C57bl6 mice. You can try aldo to radiate a Sprague-Dowley rat before implantation of B 16 cells, preferably the F10 clone
There is also a chance to decrease the immune response by initiating major injury for prolonged period before 3/4 weeks of insertion.
I really appreciate the answers and sorry for being off the scene for so long
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