I need the protocol of staining the islets using Giemsa stain to differentiate alpha, beta and delta cells.
NOTE: Since a have seen this request a second time now, I would like to invite prospective readers/replies not to answer in this form but to shift this one to the following thread found at:
https://www.researchgate.net/post/Protocol_for_staining_semithin_section_of_pancreatic_islet_using_giemsa_stain2, where the first reply starts 13-10-22, with:
Dear Mohamed,
guessing you are dealing with resin embedded blocks and their semithin sections (0.25- 1µm) and not "semithin = very thin paraffin- or cryosections ...
It might be necessary to define more precisely... at least in terms of the resin you have embedded your pancreatic islet-containing tissue... and, perhaps,
what the purpose of using Giemsa in your case would be essential (since there are a lot of semithin staining procedures out in the wild which in general consist of alkaline/basic dyes and can easily be applied to semithin resin sections and most interstingly, contain/use also dyes which are essential in Giemsa stain (e.g. Azure II, Methyleneblue, etc.) in classical (light microscopical) Histology-Histochemistry.
Contrary to that a "real" Giemsa Stain will not stain ordinarily on (hydrophobic) resin sections, at least unless you remove / solve /"etch" the surface of resin sections by use of highly alkaline and corrosive organic solvents like K- or Na- Methylate (saturated ethanolic solution, old-fashioned and somehow complicated making of!).
Even if the solving process has been achieved successfully this does not mean that the "Giemsa Stain true" will yield a reliable positive result.
The mentioned techniques are "old-fashioned", have been used extendedly in the 1980ies to overcome the problems of staining with "histological" staining methods (= more/less hydrous acidic dyes and their combinations) and has led some scientists to get desperate sometimes....
Not to be too long here... if you want to know more, please post here your short comment and request.
Best wishes and regards,
Wolfgang
PS: for easier and straight communication, please don't post in this form, but reply to the same question at:
https://www.researchgate.net/post/Protocol_for_staining_semithin_section_of_pancreatic_islet_using_giemsa_stain2
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