Dear Mohamed

The article below describe how can you stain the different types of cells in the pancreatic islets but i dont have acess to get it.

Giemsa Stain Applied to Deplasticized Sections to Identify Pancreatic Islet Cells

1986, Vol. 61, No. 6 , Pages 367-373

O. Díaz De Rada1, P. Sesma1, J. Lopez1, J. J. Vázquez1† and A. Ortiz De Zárate1

1Department of Histology and Pathology, University of Navarre, Pamplona, Spain

†Correspondence: J. J. Vázquez, Department of Histology and Pathology, University of Navarre, 31080, Pamplona, Spain

Abstract

A new application of the Giemsa stain to demonstrate endocrine cells in deplasticized sections of Epon embedded material is described. Its application to the pancreas of Rana temporaria is illustrated. The technique does not require postfixation with OSO4 and is easily performed in 30 min. It allows the easy identification of three types of endocrine cells (A, B, and D). A cells, preferentially located at the islet periphery, stain purple-blue. B cells, which occupy the interior of the islet, display a lilac color. D cells give a strong purple color, they are located both in the periphery of the islets and scattered among acinar cells. Positive identification of the cell types was made by immunocytochemistry and electron microscopy.

Best regards

Thanks for your reply . I know about this article but i don't have excess too