How to stain ROS in Arabidopsis seedlings by H2DCFDA ?

06 February 2017 1 5K Report

Dear scientists, I need your suggestion who are working with monitoring ROS level in arabidopsis seedlings by using H2DCFDA. ..

I am facing trouble to optimize the concentration(molar) of H2DCFDA, which is the right concentration and how to incubate ...

I am incubating with 10uM H2DCFDA in dark for 10 min.

Your suggestions are really needed....

Tori

Vijay Yepuri

Please find the enclosed attachment might be useful.

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