Hi,
What strategies are there to solve structure of a complex containing several proteins using molecular replacement if there is no structure for the complete complex?
Thanks
Hi Oleg,
first of all, is there similar (~60 % or more) structures already solved for each protein of the complex?
it would help if you could estimate how many monomers of each protein composes the complex and how many complexes composes the asymmetric unit (take a look at Matthews coefficient for number of amino acids in the asymmetric unit).
Using Phaser you can add different structures and ask to look for determined number of each one in the asymmetric unit. Others programs are able to do it as well.
Perhaps you can find initial phases even if you don't add one of the protein members. Depending on how the other ensembles are similar to your proteins and/or how representative this missing member is in your complex.
Good luck
Agree with Marcelo.Depends from which models of individual components of your complex do you have. In my case I used Molrep. You have to start from one protein,find it, fix and trird to find another protein...Good luck
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