What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

I.P. drug injection- time to reach brain?

I am injecting different serotonergic drugs into mice i.p. and see an effect in the brain electrophysiology quite fast, i.e. within minutes, when using a specific receptor agonist drug. I do not...

01 February 2021 8,406 3 View

When testing for outliers in a moderation analysis with a dichotomous moderator, is the overall sample size used to adjust Cook's and S.residuals?

This managed to throw me for a loop. When I'm assessing the presence of outliers before I conduct my moderation analysis I use SPSS to split the data file based upon the dichotomous moderator. I...

24 August 2020 6,578 3 View

Hello, does anyone have any links/resources for an extraction method of melatonin dissolved in AQ solution?

Hello, I currently have a product that has a small amount of melatonin dissolved in it (aqueous solution), and I need a sufficient amount to get a reading on an IR spec. I was thinking of...

04 December 2018 7,975 3 View

Does anyone have a protocol for the extraction of nuclear and cytoplasmic RNA from plant leaf tissue?

Hello, I'm looking for a protocol that will allow the extraction of nuclear and cytoplasmic RNA from the same plant leaf tissue sample (Arabidopsis thaliana). I have found protocols for the...

16 August 2018 158 2 View

Other than HPLC, is there a good method to measure intracellular retinoic acid (RA) concentrations in the cytosol of cultured cells?

I have come across an ELISA to measure RA levels in serum/plasma, however I am not sure if this sensitive enough to determine RA concentrations in cell lysates (0.625-10 ng/mL). I was hoping...

10 October 2017 2,650 2 View

Does anyone know a good way of getting plasma from frozen whole blood?

11 July 2017 9,233 4 View

How to prevent background DIG staining in in situ PCR method?

I am performing in situ PCR of an RNA target in bovine lymph tissue; incorporating the DIG-dUTPs directly in the PCR product and then detection via anti-DIG Fab AP binding and subsequent...

08 February 2016 5,716 3 View

Are item means typically different in Xcalibre?

I am running an IRT analysis on an instrument in XCalibre, and the analysis reports substantially different means for the items than those calculated in Excel? Is there some weighting happening of...

30 January 2015 7,946 3 View

Is there a way to use immunohistochemistry to show changes in metabolism of murine muscle tissue following schema (glucose vs lipids etc)?

I am using the hind limb ischemia model in NOD/SCID mice and would like to measure changes in metabolism to the localised tissue both in a control mouse and mouse with recovered hind limb...

02 July 2014 7,096 1 View

How to analyze a particular region of my simulation box in GROMACS?

Hi, I am using GROMACS 5.1.4 to simulate water in contact with a surface. I am only interested to calculate some properties of water near the surface. Let's say at a distance of x nm from the...

03 March 2021 9,989 2 View

Build an orthogonal box in ASE?

Hello, I was wondering the best way to build an orthogonal box filled with a defined crystal structure in ASE. I know it could be easily done in LAMMPS, but I guess it is also doable in ASE. The...

21 February 2021 5,899 4 View

How to obtain regions around the person bounding boxes(excluded the detected people in scene)?

Hi all. I have a set of coordinates of bounding boxes for AVA Dataset. This dataset is used in action recognition. My goal is to get the areas around the people (people are inside boxes) and do...

13 February 2021 2,538 2 View

How to deal with multidimensional predictor in multiple regression?

I am investigating the influence of Trait Emotional Intelligence(TEI) and Communication styles (specifically Precision and Expressiveness styles) on Academic performance. I am now in a fix as to...

10 February 2021 7,630 3 View

GROMACS: how do I know how many box images a particular atom has moved from from t=0 to t=N?

In GROMACS how do I know how many box images a particular atom has moved from from t=0 to t=N? LAMMPS, for example, has the option to include image flags in the dump file for x, y, and z. Is there...

30 January 2021 8,929 3 View

Why can I not enter the title of the journal?

When I upload articles the details page does not allow me to enter the names of lesser known journals. It says "type to search" but because the journal is not on the list, I am left with a blank...

26 January 2021 2,988 3 View

Antibody storage containers?

Does anyone have suggestions for opaque antibody storage boxes (ideally not cardboard) that can hold variable sizes of antibodies. We are getting by with foil wrapped tackle boxes but I was hoping...

25 January 2021 2,591 4 View

AMBER tleap solvatebox always undervalues the density, why?

I've been searching for an answer to this question for years, and I'm shocked I haven't found any discussion on any forums. Here is an example to provide more details: I am preparing a 450...

20 January 2021 7,561 2 View

When reporting mediation in a manuscript being submitted for publication, what have you found to be the easiest way to create your path diagram?

I am most familiar with using text boxes in WORD but I wasn't sure if that would be acceptable.

11 January 2021 3,585 1 View

How would I go about equilibrating the internal humidity of a closed system at 2 different temperatures?

I have a box which is kept at 4C which is enclosed in a ziploc bag. The dimensions of the box are: 18cm x 13.4cm x 4.9cm. The relative humidity of this internal environment is 30% at this...

07 January 2021 6,734 3 View