Model specification for moderation in multilevel model with R?

I've got longitudinal data from a psychological experiment and try to model the data using R package lme4. My dependent variable y is continuous and is measured at eleven occassions. Each...

13 April 2020 6,619 4 View

How to connect hypothesis to your discussion?

Hello, The question was answered. Thank you! Kind regards

12 January 2020 1,834 2 View

Convert units of pre-exponential factor in Arrhenius equation for reaction rate?

I found several pre-exponential factors for the reaction rate of calcium carbonate crystallization. They are usually given in m^4 kg^-1 s^-1. Is there any change I can convert them to m^4 mol^-1...

08 October 2019 3,770 1 View

Is it true that light produced in the core of the sun needs a million years to reach the surface?

Atoms in the sun core are very fast moving. I read, that for this reason the light produced as a by-product of hydrogen fusion cannot pass directly to the sun surface (which would be a matter of...

19 January 2019 3,495 3 View

If we look from Earth into deep space with our telescopes beyond our galaxy, is the universe uniformly dense in all directions?

26 January 2018 2,314 5 View

Best way to clean CTFE equipment in order to avoid DNA contamination?

Dear colleagues, I received different pieces of lab equipment (mainly pestils and alike) out of CTFE and would like to reuse the same pestils without autoclaving them every time. I do not have any...

29 August 2017 4,356 2 View

What shall I do, when I conducted an exploratory factor analysis and it doesn't identify two of my constructs as separated constructs?

I conducted an explanatory factor analysis (I used different rotations and used principal component). The explanatory factor analysis wasn't able to identify two of my model's constructs as...

25 July 2017 8,445 2 View

Has anyone observed that MIN6 progressively express less GLP1R with increasing passage number?

19 October 2016 3,228 1 View

(Spatial) Working memory task in headfixed mice ?

I am investigating (spatial) working memory in mice. Due to the nature of the project the mice are head-fixed and therefore are not able to move around freely, eliminating most common tests for...

04 July 2016 2,997 2 View

What are best practices and relevant tools of sustainable financial investments (& insurance meachnisms) in rural activities and/or landscapes?

In many rural areas of "developing countries" small scale agriculturalists (farmers/fishers/herders/...) see the necessity for (financial) investments, they have the capacities (social & human...

25 June 2015 5,367 5 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

Why initial concentration of Pb not the same as calculated one when testing using AAS?

I am currently doing research on the adsorption of heavy metal using magnetic reduce graphene oxide. I use AAS to determine the concentration of the heavy metal. I prepare the Pb solution with...

26 February 2021 9,580 4 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Diltuions for a Western blot?

I need help using the equation C1V1=C2V2 figuring out how much antibody to add to use for a western blot. The stock was origianlly 3.45 ng/ul but diluted 1:10 so now its 0.345 ng/ul. I need to run...

22 February 2021 9,901 3 View

What are alternatives for B27+ Supplement in neuronal cell culture?

Hello everybody In our iPSC-derived neuronal culture we use Maturationmedium, supplemented with B27+. As Thermo Fisher run out of stock and can deliver earliest in May, I wanted to ask whether...

16 February 2021 3,348 1 View

Can 5-FOA Diffuse Through Agar?

I am preparing to do a gene knockout in the filamentous fungi Coccidioides posadasii targeting pyrG/URA3 so that 5-FOA can be used for selection pressure instead of traditional antibiotic...

16 February 2021 169 4 View

How to revive a Hyphomicrobium strain?

11 February 2021 3,524 2 View