I am designing LAMP primers for detection of a bacterial gene using Primer Explorer. Five primer sets were generated, 2 discarded due to high dG. None of the remaining 3 sets generates LF but only LB loop primers. Am I missing anything? Same sequence gave me loop primers with Primer Designer software. I will appreciate sharing in your experience and knowledge please. Thank you in advance.
Gabriel Avila , I adjusted my primer design parameters in the software to allow for less stringent conditions. I got primer sets with complete loop primers by doing so. Hope this helps? Thank you.
Dear, I will try to adjust my parameters. I hope that this problem can be solved! Thanks for your answer
Number of Primer Candidates: LF=0, LB=30
Filtering by dimer-dG.
6 Primer piece(s) were generated.
PLEASE I NEED HELP WHAT SHOULD I DO TO SOLVE THIS PROBLLEM
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