Why do we need to neutralize the charge of the system by adding ions before we proceed to energy minimization step in GROMACS ?

I am learning MD simulations using GROMACS and while I was doing a protein in water MD simulation a doubt raised. I was following the steps mentioned on the tutorials page and it said The system...

15 February 2021 5,514 4 View

Divergence detected in AMG solver for temperature and species , DEFINE_VR_RATE, Fluent ?

In 2D Model, Reaction in porous Zone, Error occurs on hooking UDF , DEFINE_VR_RATE in Fluent, .The error spikes just after running calculation after 1st iteration. Divergence detected in AMG...

16 November 2020 9,053 3 View

How to verify if a measuring system is working right?

We have 2 machines that test products. Both of these qualify the product to be Good or Bad ( Based on amount of leak ). The final outcome is attribute variable (Good/ Bad) whereas the way we...

16 November 2020 688 7 View

How to create a surface plot and contour? If i have an excel file with X, Y ,Z values of the surface ( Periphery of a tube) and Q (heat flux) value.?

Need to create 3d surface plot. Data I have X, Y ,Z coordinate of the pipe surface and the corresponding Q (heat flux) values at that point. Nearly 222440 data points of a 2m length pipe. Thank you

07 October 2020 9,854 7 View

Initialization in Fluent? Hybrid initialization or Standard initialization ?

Looking towards an incompressible flow (with K-e, turbulence model), with mass flow/velocity inlet and Pressure outlet boundary condition. Which initialization method preferred? How far it can...

05 October 2020 4,742 5 View

Why did it take humanity so long to start writing though we have been speaking for tens of thousands of years ?

They say we were just speaking and not writing anything for tens of thousands of years till a basic form of writing evolved just about ten thousand years back. Why this yawning gap ?

22 September 2020 5,313 7 View

Which is the best software to simulate chemistry of materials in correlation with their mechanical properties ?

ANSYS or ABAQUS donot correlate materials chemistry wirh the observed or simulated mechanical properties. Is there a software that can do this structure to property correlation. ?

22 September 2020 1,171 3 View

Is there any cost-effective, simple and efficient way to analyse and estimate the amount of vitamins in algal samples?

I am currently pursuing my research in Phycology. I'd like to know if there's any simple and cost-effective method to detect and analyze nutrients and vitamins in the algal matter. The experiment...

18 September 2020 4,081 3 View

Which research institutes in India have an algal identification facility?

I am currently pursuing my research in Phycology and planning to conduct a study on microalgae. Is there any place where I can send my samples to be identified? (Preferably in South India) Also,...

17 September 2020 4,440 2 View

How is the co-expressed genes regulatory network plotted? Is there any Bioinformatics tool for the same?

Hi, I am working on molecular validation of co-expressed genes in Rice under moisture stress. I have a list of genes that are co-expressed in three rice varieties, under a particular set of...

12 July 2020 7,494 1 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

For modelling epoxy-based matrices (in composites), which model in ABAQUS is the most suitable, Concrete damaged plasticity or Drucker-Prager model?

I am working on developing a micromechanical FE model for predicting kink-band formation in UD composites. To model matrix plasticity, which model, out of Concrete Damaged Plasticity and...

28 February 2021 5,415 1 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Are there lab markers/pens resistant to Roti-Histol?

Hi, I'm looking for a pen/marker to write on the smooth, transparent (non-frosted) part of microscopy slides which does not get washed away by alcohols and most importantly Roti-Histol. I want to...

24 February 2021 9,902 5 View

Quantifying leaking DNA from bacteria - what's the secret?

Hi, all! I need to determine if my treatment causes membrane damage in bacteria (S. aureus and E. cloacae), but I cannot use a fluorescent probe such as PI and similar ones because my compound is...

24 February 2021 2,006 2 View

How the Ground Plane implantation is performed without damaging (and w/o varying the undoped nature) the top Si (8-10 nm) in FDSOI technology ?

It is a general practice to introduce Ground Plane in FDSOI devices under the BoX. The Ground Planes are introduced by Ion Implantation through Top Si layer and Buried Oxide. I wanted to know that...

24 February 2021 5,974 4 View

How to Control Whiteflies in coconut leaves?

24 February 2021 1,005 1 View

Any references about insects or animals that can damage or weathering of rock or fossil material?

Are there any references to insects or animals that can cause damage or weathering to the rock or fossil material? books or paper that would be nice. Thank you very much!

24 February 2021 6,805 3 View