"Ability to make friends make person glide instead of stumbling through life". How much do you agree with the statement?

How ability to make friends matters in today's busy life style? What are positive effects of friendship on a human being?

10 November 2017 1,005 10 View

What is brain washing? What is it's scientific significance?

What is scientific description of brain washing? How it is possible for and adult?

10 November 2017 7,105 3 View

What role love and affection play to control happiness in our life? Is there any substitute of feeling of love and affection?

Up to what extent feeling of love and affection provide energy, activeness and happiness in human life? Is there any substitute of feeling of love and affection?

10 November 2017 4,765 5 View

What can be model way of living each day for a scientist?

How scientists can make their each day more devoted for society and self happiness? What can be model way of living each day for a scientist?

10 November 2017 7,700 1 View

What are core values of a scientist? How to protect core ethical values in present changing environment?

What are core values of a scientist and professionals? How to protect core ethical values in present changing scenario ?

10 November 2017 738 10 View

Is it possible to reprogram human personality? If so to what extent?

We see many advertisements for personality development program. Is it true to develop personality by external training? If so to what extent?

10 November 2017 7,132 6 View

What is happiness? How it affects human personality?

How level of happiness can modify behavioral responses?

10 November 2017 3,168 2 View

How to view self published research articles list of SCI Indexed Journals on (ISI) Web of Knowledge ?

It is easy to view published SCOPUS indexed article list. How to view self published research articles of SCI indexed Journals on ISI Web of Knowledge?

09 October 2017 9,410 5 View

Who will be called best ruler?

What qualities of ruler are appreciated by public?

09 October 2017 4,588 0 View

How to increase accuracy of a micro controller based sensor system?

How to design higher accuracy micro controller based sensor system?

09 October 2017 2,879 1 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Can cells be reused after using WST 8/ CCK 8 from Sigma Aldrich?

Hi all, Can cells be reused after I have seed them onto 96 well plates and test them with cell counting kit at OD450 (CCK8) at 12 hours, wash them with PBS x3 and add new media and let them...

24 February 2021 5,072 1 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

What is the standard protocol in doing replicates for C. elegans lifespan assay on solid media (thru worm picking)?

I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...

23 February 2021 9,933 1 View