Dear community, I need to run a J-HMBC experiment in a Bruker spectrometer and the pulse program (hmbcetgpjcl2nd) does not appear in the parameter set window (after rpar); however, I can set the pulse program from the ACQUPARS tab -->PULPROG. When running the pulse program, it does not give me any spectra, just noise. How can I optimize this pulse prograam to work properly? It is obvious there is something I am missing (I have never done this before).
Thank you very much for your time and knowledge generosity!
Carlos
You can find some comments about the required parameters at the end of the pulse program. Pay particular attention to the gradients, constants (cnst6, 7 and 13 respectively) shaped pulses and FnMODE.
You probably want to start from something like the HMBCETGPL3ND parameter set, which already has most of the needed parameters.
Thank you, Jani! I followed your advice and got a pretty good spectrum. Many thanks for your time!
Dear Carlos,
Yes Jani Rahkila is correct. You can unse the parameterset HMQCETGPL3ND it contains the pulseprogram hmbcetgpl3nd. I think the only difference to the one you inteded to use is a 3-fold instead of a 2-fold low-pass J-filter to suppress one-bond correlations. I am not an expert in this, since I am doing solid state NMR, but this should work too. But no garantee.
Alternatively you could set up the parameters youselve , like Jani Rahkila said, as described in the pulseprogram (you can open it in the pulseprog tab clicking at E).
Gennerally there are 2 ways to setup a new experiment. Ether you load a parameteset, which contains the pulseprogram and the corresponding parameters (which are not depending on your type of instrument) and than perform getprosol loading the instrument (probe) dependant parameters. Or you choose or write a pulse program and than setup the parameters yourselve in the ACQUPARS tab including or excluding getprosol.
Many Greetings,
Kristof
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