I would like to build a lateral projection of a z-stack with the correct linear size ratio for the final illustration, how can I change the pixels size ratio in ImageJ?
Dear Borys Olifirov
These links might be useful, have a look:
1. https://imagej.nih.gov/ij/docs/tools.html
2. https://imagej.nih.gov/ij/docs/guide/146-30.html
3. https://imagej.nih.gov/ij/docs/menus/analyze.html
4. https://imagej.nih.gov/ij/docs/guide/user-guide.pdf
Kind Regards
Qamar Ul Islam
If you want to just reset the pixel size to micrometer:
Get the pixel size/micrometer information from the confocal metadata (when you open your images using ImageJ or FIJI it should automatically populate the information)
When the image is open:
Click Image on the tab > Click Scale (Control+E on FIJI) > A Scale window will pop-up. On the Scale window you can set the X, Y or Z scale accordingly and also modify the Width and Height pixels.
If you are talking about deconvolution and PSF you can also perform those using ImageJ or FIJI.
Good Luck!
@Sathya Srinivasan
You can also click on > Analyze tab > Click Set Scale and reset the scale (this is to convert your pixels to microns where you should have a scale bar of known size).
Sathya Srinivasan
- Badges
- Science topic
- Similar topics
- Biological Science
- Bioecology
- Systems Ecology
- Landscape Ecology
- Connectivity