We followed the setup instructions and tutorials for the Axoclamp 900A and successfully tested the system with the model cell. We are now preparing for actual recordings in Xenopus laevis oocytes.

• When we clamp Channel 2 at a holding voltage of -60 mV, the system reports a voltage of -126 mV and a current of -60 nA. Why is the recorded membrane potential more negative than the command voltage? Is this discrepancy normal during model cell testing or does it suggest a configuration or calibration issue?
• We tried mounting the electrodes and immersing them in the bath solution. How can we measure or verify the access resistance of the electrodes directly from the software? Is there a specific procedure or command we should run to get this value?