I have done the NLC formulation using ultrasonication and microemulsion techniques. Now I have the whole emulsion as a milky white liquid. How to separate the NLC from the aqueous solution. I have done a single centrifugation. I assumed that the pellet had the NLC and the supernatant had the free drug. And I have to spin the emulsion at 25,000 rpm for 10 minutes. But the pellet hasn't completely settled. So please suggest a protocol to separate the NLC from the aqueous phase
Before I can help I need afew clarifications:
What is NCL
Have you made a microemulsion? If yes what surfactants did you use?
You make frequent references to pellets I assume you mena particles
G. Bognolo.
NLC= Nanostructured lipid carrier;
surfactants used: span 80 and tween 80
As referred in papers the nano emulsion should be centrifuged at 25000 rpm for 20 minutes so that the nano sized particles settle down at the bottom
Microemulsions are thermodnamically stable, so if you want to break one you have to use pressure or temperature. Have you tried to heth or cool your emulsion? Mind you, when the emulsion breaks you loose the identity of the particle. If you want to just separate the nanoparticles and preserving their identity gravity separation is the only way. I seem to understand that you have already used ultracentifugation with some success. The other possibility is to build agglomerates of larger particles that should settle more quickly. If your system can tolerate you may try low concentrations of di or trivalent cations. Start with say 0.001 molar CaCl2 or AlCl3. If nothing happens go to higher concentraations,
Best regards
G Bognolo
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