hello,

I think you can use TCA (acide trichloracétique),

TCA it used for TBARS test to deproteinize the cell in order to calculate only lipid peroxidation.

see this paper may help you

Article Identification of Bovine Sperm Surface Proteins Involved in ...

Proteins from the entire spermatozoa werecan be extracted by taking around 60 million sperms in 100µL of lysis bufer containing 4% CHAPS, 40mM Tris-base,

75mM DDT, 1mM PMSF, 1mM EDTA, 7mM urea, 2mM thiourea, 1mM sodium orthovanadate and protease

inhibitor cocktail @10 µL/mL. Te mixture was incubated at room temperature for 60 min followed by centrif-

ugation at 21130 g for 30 min at 4°C. Afer centrifugation, the supernatant was separated and stored at −20 °C

for further use. Protein concentration was determined by Quick Start™ Bradford Protein Assay Kit (BioRad,

Hercules, USA).Also if you do acetone precipitation very nice bands would come.....