How to separate PBMC from Keratinocytes on a plate with collagen coating?

16 June 2021 1 4K Report

Hello,

In my research I grow Keratinocytes in 96well plate using collagen coating matrix.

I need to check the influence of PBMC on the Keratinocytes, but when I add PBMC onto the plate it sticks to the collagen too.

How can I grow PBMC and Keratinocytes and then separate them?

Thank you!!

Steingrimur Stefansson

Hi Nofar Matana . If the cells don't need to be in physical contact, you could look into Boyden chambers that keep cells separate but allow media interaction.

More Nofar Matana's questions See All

CRISPR Cas9 gRNA design tools - which one to use?

Hello, I hope that if you are reading this you are doing an interesting science :) I want to design guide RNAs for CRISPR Cas9 knockout screen I'm planning (for human cells cultures and mouse...

04 May 2024 4,727 1 View

How do efficiently dissolve H3BO3 (Boric acid) in UPW?

Hello :) I am trying to prepare saturated Boric Acid in water. According to the protocol i'm using, I supposed to dissolve 100mg of Boric Acid in 200ul of UPW, vortex it, incubate it for 20...

02 July 2023 5,688 0 View

What could be the reason for not getting reproducible results in western blot?

Hello everybody, I have simultaneously made biological duplicates of the U937 cell line with some treatments. I expected to get the same results for STING pathway proteins in western blot, but to...

17 December 2022 2,324 3 View

How to choose the right housekeeping gene for RT-PCR?

Hi everyon! It's a long question, and I hope I'll be able to explain myself well. I did a RT-PCR reaction for CD11b with a housekeeping genes panel, to pick one that best suited my cells. Overall,...

18 March 2020 719 5 View

During tensile testing of elastomeric polymers, how we can avoid having sliding of the dogbone samples at the grips?

During tensile testing of elastomeric polymers, we have been facing sliding of the dogbone samples at the grips. Even we sticked double sided sand papers to the end sides of the dogbone samples...

14 April 2016 3,492 10 View

Adhesion strength of coating?

How can I determine a good adhesion strength range for coatings on polymer surfaces, such as DLC on polymer substrates? Is there a specific threshold for adhesion strength (from T-peel tests)...

10 August 2024 942 3 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

How do I conformally do PR spin coating on trench structure?

I did PR spin coating on trench structure. I used AZ P4620 PR and the thickness or PR is around 11um. The substrate is Si. And my trench structure depth is 141um(negative way). Even though I...

08 August 2024 2,298 0 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Is changing the medium during the experiment overestimate the results?

I am experimenting with cancer and non-cancer cells in a 12-well plate for 4 days with a seeding density 1*10^4/well, however, I noticed that the control group growth rate slows down on D3. Should...

07 August 2024 2,283 2 View

Why do i have a weak plasma and no coating during rf sputtering?

I have been getting a low plasma and no coating while doing rf sputtering of copper doped ZnS using a power of 140 watts and at pressure of 6.5x10-3 Mb.

07 August 2024 4,526 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Why do we equate male and female arousal?

Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)

05 August 2024 9,537 2 View