Hello :)

I am trying to prepare saturated Boric Acid in water. According to the protocol i'm using, I supposed to dissolve 100mg of Boric Acid in 200ul of UPW, vortex it, incubate it for 20 minutes in room temperature, centrifuge it and collect the supernatant.

But the problem is in the initial step, it doesn't dissolve at all.

I tried to dissolve it in warm water (100C) with no success, and also vigorous vortexing is not helping (and also hit block with vortexing didn't help).

I'm using Boric Acid Sigma-Aldrich, cat. no. B0394 - ACS reagent >= 99.5%.

If you have any tips from your experience I would be glad,

Thanks in advance,

Nofar :-]

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